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Tuesday, May 12, 2020 | History

2 edition of Tissue culture propagation of Dendrobium. found in the catalog.

Tissue culture propagation of Dendrobium.

Djunaedi Gandawidjaja

Tissue culture propagation of Dendrobium.

by Djunaedi Gandawidjaja

  • 5 Want to read
  • 36 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (M.Sc.) - University of Birmingham, Dept of Plant Biology.

ID Numbers
Open LibraryOL20661468M

ADVERTISEMENTS: Read this article to learn about the plant tissue culture. Its benefits, structure, types, techniques and applications. Plant Tissue Culture: Plant tissue culture broadly refers to the in vitro cultivation of plants, seeds and various parts of the plants (organs, embryos, tissues, single cells, protoplasts). The cultivation process is invariably carried out in a [ ]. The invention discloses a method for greatly propagating dendrobium candidum round bulb by utilizing airlift bioreactor. 2L liquid culture medium is injected into a 3L bioreactor, 20g round bulb is inoculated, when luminous intensity is Lux and air injection amount is vvm, completely deep culture is carried out for 30d, fresh species can reach g, thus being applicable to Author: 廉美兰, 朴炫春, 孙丹, 尹成日.

Tissue Culture. Tissue culture, or micropropagation, is the standard commercial way of cloning orchids. It's hard to do at home because you need sterile conditions.   Abstract. A commonly held view is that the ideas and basis for the practice of orchid micropropagation arose de novo in from the work of Georges Morel in France. In this paper we argue that the crucial developments in micropropagation were made by Gavino Rotor in in the USA and Hans Thomale in in Germany, and that Morel's work needs to be seen in the context of a Cited by:

This volume presents a compendium of methods for the multiplication of orchid plants by the use of tissue culture techniques. These techniques are at the forefront of plant molecular biology research and have proven to be of great practical utility for growers of rare orchids.   Dendrobium hookerianum is a rare and threatened epiphytic orchid of northeast India. Prospects for conservation would be strengthened by developing an in vitro method for mass propagation. Seeds are minute and difficult to use directly in the field for this purpose, being non-endospermous with a low nutrient content and dependent on a specific fungus for germination and Cited by:


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Tissue culture propagation of Dendrobium by Djunaedi Gandawidjaja Download PDF EPUB FB2

TissUe cUlTUre Meristem cul-ture, a type of micropropagation by tissue culture, is the standard method for mass propagation of the most popular orchids. the ever-popular phalaenopsis, for exam-ple, are normally propagated through tissue culture, because vegetative propagation of these orchids is slow, variable and difficult.

Propagation of orchids There are several ways to propagate orchids. Propagation from seed or sexual propagation Pollen is used to pollinate a flower. When the seed pod matures (which may take Tissue culture propagation of Dendrobium.

book months), the tiny seeds can be sowed. Not all seed pods will File Size: KB. Advancement in Tissue Culture of Orchids. Plant propagation by tissue culture. Protoplast i sola tio n and culture o f Dendrobium Son ia “Bom 1 7”. tissue culture of Dendrobium and Darwinara, sorbitol and mannitol were detected in the medium following one m onth PLB micropropagation (Kishi an d Takagi b).

The primary objective of a plant tissue culture experiment remains in the production of maximum number of proliferating shoots during clonal propagation. Although cytokinins have a wide array of functions, their significant regulatory role on different physiological and developmental processes in plants is well documented (Werner et al., ).Cited by: An efficient in vitro regeneration protocol from seed culture has been established successfully for Dendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal values.

Seed germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins. Medium supplemented with % activated charcoal (AC), Cited by: 9. Chapter II INTRODUCTION TO PLANT TISSUE CULTURE Plant tissue culture is one of the biotechnological tool used in the mass propagation of high value crops, specially orchids.

An efficient in vitro regeneration protocol from seed culture has been established successfully for Dendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins.

Medium supplemented with % activated charcoal (AC), 2 mg/L 6-benzyl Cited by: 9. Vanilla (for spice) and Dendrobium sp. (as cut flower) have been recognized as two of the priority plants for tissue culture propagation according to a report prepared on market survey on tissue cultured plants (Biotech Consortium India Limited for Department of Biotechnology (DBT) and Small Farmers’ Agri-Business Consortium, ).Cited by: Plant Tissue Culture is the process of growing isolated plant cells or organs in an artificial nutrient media outside the parent organism.

In other words, it is an in vitro culture of plant cells or tissues on artificial nutrient media under aseptic conditions, in glass containers. This is a technique by which new plants can be raised on artificial nutrient media by the use of plant parts or. Photoautotrophic tissue culture is defined as "micropropagation without sugar in the culture medium, in which the growth or accumulation of carbohydrates of cultures is dependent fully upon photosynthesis and inorganic nutrient uptake".

There are multiple advantages to using this form of propagation, because this system actively encourages plant growth.

Tissue culture has now become a well-established technique for culturing and studying the physiological behaviour of isolated plant organs, tissues, cells, protoplasts and even cell organelles under precisely controlled physical and chemical conditions.

Micropropagation is one of the most important applications of plant tissue by: 8. - Explore adina's board "Plant tissue culture" on Pinterest. See more ideas about Plant tissue, Culture and Plants pins. Vanilla (for spice) and Dendrobium sp. (as cut flower) have been recognized as two of the priority plants for tissue culture propagation according to a report prepared on market survey on tissue cultured plants (Biotech Consortium India Limited for Department of Biotechnology (DBT) and Small Farmers’ Agri-Business Consortium, ).

Micropropagation of Orchids, 2nd edition, is a valuable book of reference for people working in the area (Thaiszia Journal of Botany, ) "This title comes as two separate hardcover volumes, attractively housed together within a 5-sided cardboard casing that matches the volume covers.

Tissue (Meristem) Culture of Orchids Propagation Equipment; greenhouses, hotbeds, cold frames, mist systems, furo light boxes, etc Cymbidiums And Dendrobiums. Cymbidium culture Dendrobium Types -soft cane, hard cane, black haired Dendrobium Culture Dendrobium species Cattleyas, Vandas And Other Commonly Grown Orchids.

Cattleya varieties and culture. Abstract. Methods for clonal propagation of the two major morphological groups of orchids, i.e., sympodials and monopodials, are presented.

The first group, sympodials, includes such genera as Cymbidium, Cattleya, Dendrobium, and are characterized by a multibranching rhizome that can supply an abundance of axillary shoots for use as by: face the extreme danger of extinction due to over-exploitation and habitat loss.

Plant tissue culture could be one of the most suitable alternative tools to minimize the pressure on natural population of medicinal orchids and their sustainable utilization.

Key words: Medicinal, orchids, propagation, conservation, culture. INTRODUCTIONFile Size: 1MB. BALL E.A. & ARDITTI J. Node cultures as a means of clonal propagation for Dendrobium.

Proc. 8th World Orchid Conf. HARNEY P.M. Tissue culture propagation of some herbaceous horticultural plants. in D.T, Ellis B.E., Harney P.M., Kasha K.J.

& Peterson R.L. (eds.) Application of Plant Cell and Tissue Culture to. Background and aims. Dendrobium hookerianum is a rare and threatened epiphytic orchid of northeast India. Prospects for conservation would be strengthened by developing an in vitro method for mass propagation. Seeds are minute and difficult to use directly in the field for this purpose, being non-endospermous with a low nutrient content and dependent on a specific fungus for germination and Cited by:.

Meristem Tissue Culture: American Orchid Society - Meristem culture - 72 pages. 0 Reviews. From inside the book. What people are saying - Write a review. We haven't found any reviews in the usual places. Contents. 3: Clonal Multiplication of Cymbidiums through Tissue Culture of the Shoot.

2 other sections not shown.Bibliography Includes bibliographical references and index. Contents. Preface Preface to the Second Edition Preface to the First Edition; Chapter 1: History Terminology Origins of Orchid Micropropagation Plant Hormones and Propagation Additives of Plant Origin Culture of Tissues and Organs Early Tissue Culture Attempts Culture of Stem Tips First Micropropagation of Orchids The Second Aseptic Author: Yam, Tim Wing.- Explore mirlen's board "Plant Propagation Science", followed by people on Pinterest.

See more ideas about Propagation, Plant tissue and Plants pins.